Portfolio Archive - Arcadia UMC


The PamStation is a fully automated CE marked instrument designed for processing a maximum of three PamChip microarrays simultaneously. PamGene’s functional kinase assays are activity-based and detects protein kinase activity directly in cellular and tissue lysates, through measuring peptide phosphorylation by protein kinases.

With PamGene’s kinase activity profiling platform, the activity of kinases in a wide range of cells and tissues can be measured in real time. We use our proprietary 3D peptide microarray technology (PamChip® and PamStation®) which offers a multiplex method for global kinase activity profiling. The assay is very sensitive, requiring only a small amount of lysate to measure the activity of kinases in various samples including cell lines, xenografts and human tissues. Lysates obtained from a few thousand cells can suffice to obtain a kinome profile of the multiple kinases present in these samples. This is accomplished by incubating the sample lysates across peptide substrates immobilized on the 3D surface of the PamChip® microarray (196 protein tyrosine kinase (PTK) or 144 serine/threonine kinase (STK). Kinases present in the lysates will phosphorylate the phosphosites, which are visualized using fluorescently labelled antibodies. Reaction times are typically minutes to an hour with only small amounts of protein input (0.5 to 5 μg per array), making it more sensitive than alternative approaches.

PTK Assay

Kinase(s) in the sample (recombinant, cell or tissue lysate) actively phosphorylate substrates on the PamChip®, in the presence of ATP. A FITC-conjugated PY20 antibody, present in the reaction mix is used to detect the phosphorylated Tyr to quantify the phosphorylation signal. Images are taken every 5 minutes to generate real time kinetics data.

STK Assay

Kinase(s) in the sample (recombinant, cell or tissue lysate) actively phosphorylate substrates on the PamChip®, in the presence of ATP. An antibody mix is used to detect the phosphorylated Ser/Thr, and a 2nd FITC-conjugated antibody is used in a detection mix to quantify the phosphorylation signal.

The combined list of detectable kinases can be found here:

Download the combined list

Assay Workflow:


Functional assay for determining full length kinase activity.


How can we assist you?

Learn about The Pamstation, and how we can assist you in your specific project.


With the Biomark™ HD system, you get reliable production-scale throughput and exquisite single-cell sensitivity, with a wide variety of sample types and chemistry choices to accommodate the most genomic applications. Fluidigm integrated fluidic circuits (IFCs) empower life science research by automating PCR reactions in nanoliter volumes. This means using less sample and reagent, and a single microfluidic device, to achieve the high-quality, consistent results your work depends on.

The Biomark HD system is the only multipurpose real-time PCR system that performs genotyping, gene expression profiling, quantitative real-time digital PCR (qdPCR), and single-cell analysis.


The Biomark HD is a flexible and high-throughput platform that enables system-wide biology. Now you can run an entire project from research, through validation and on to routine screening in a single system without compromising speed or data quality.

Assay flexibility

Biomark HD workflows are compatible with TaqMan® Assays and reagents as well as a number of other commercial assays, including Fluidigm SNP Type™ for genotyping and Delta Gene™ for gene expression. Easily move your existing panels to the Biomark HD to achieve immediate cost efficiencies or take advantage of commercially available panels including the Advanta™ Sample ID Genotyping Panel and Advanta IO Gene Expression Assay.

A fast, scalable and cost-efficient platform that supports multiple applications, Biomark HD is an ideal solution to achieve higher productivity.

How can we assist you?

Learn about our Biomark HD services, and how we can assist you in your specific project.


Since March 2020 Arcadia is partnering with Biotechne to provide services for immuno-assay testing and development supplying you with ELLA Next Generation ELISA technology.

ELLA enables the detection of biomarkers in single and multiplex formats in a microfluidic setup. Ella’s combination of performance and workflow brings immunoassays to the next level delivering data with sub-picogram level sensitivity, 4+ logs of dynamic range and reproducibility that rivals the best laboratory automation. Ella’s assays support single or multianalyte experiments with no crossreactivity, and getting good immunoassay reproducibility. Ella provides the analytical power for your research with a high level of automation and validation in a wide variety of application fields like Cancer, Neurology and Cell Therapy. 

Biomarker discovery and validation experiments can be executed on multiple types of samples ranging from serum and plasma to CSF.


How can we assist you?

Learn about our Ella services, and how we can assist you in your specific project.


As CRO partner for Meso Scale Diagnostics we provide services for immunogenicity testing, multiplexed biomarker analysis, and custom assay development. The MSD technology enables the detection of biomarkers in single and multiplex formats utilizing ultra-sensitive electrochemiluminescence detection. A wide product line include many single and multiplex assay kits for profiling biomarkers, cell signaling pathways and other applications. In addition the open platform format allows for custom assay development. 

Increasingly, studies have shown that many biomarker levels are actually lower than previously thought, we are excited to be able to offer you the new S-plex format assays that can offer you unmatched ultrasensitive biomarker detection spanning the fg/mL range. This will open applications for: biomarkers that are believed to be present, but are not measurable; biomarkers that are known, but below detectable limits at native levels; small changes in biomarkers related to disease progression.


How can we assist you?

Learn about our MSD multi-array technology services, and how we can assist you in your specific project.


Utilizing xMAP technology for multiplex analysis of your biomarkers within a single sample. With our Luminex 200 system Arcadia offers Multi-Analyte Profiling (xMAP) technology allowing simultaneous analysis of up to 100 bioassays from a small sample volume, by reading biological tests on the surface of microscopic para-magnetic beads. xMAP technology combines this miniaturized liquid array bioassay capability with small lasers, digital signal processors, photo detectors, charge-coupled device imaging and proprietary software to create a system offering advantages in speed, precision, flexibility and cost. 

xMAP technology is currently being used within Arcadia for various research projects, ranging from biomarker discovery and validation in large pre-clinical cohort studies as well as assay development. To facilitate researchers in biomarker discovery and validation, ARCADIA offers a ISO9001 work environment with a wide range of cuttingedge technology platforms suitable for measuring several markers simultaneously in low sample volumes such as OLINK (Targeted Proteomics) and multiplex assay platform like MSD Discovery, Ella and Luminex.


How can we assist you?

Learn about our Luminex services, and how we can assist you in your specific project.


The ARCHITECT ci8200 (Abbott) is a large load capacity instrument which can be found in a high number of clinical core laboratories around the world. It provides clinical chemistry and immunoassay testing in a single integrated system. By delivering fast, high-quality STAT results, the ARCHITECT ci8200 can handle complex workloads.

The ARCHITECT ci8200 integrated system provides a single testing module that is specifically designed to improve laboratory efficiency and turnaround time. It offers a maximum throughput of up to 1400 tests per hour, including 1200 clinical chemistry and 200 immunoassay tests. Featuring a load-up capacity of 365 samples with 35 priority positions. In addition it has up to 146 refrigerated reagent positions plus
Integrated Chip Technology (Na+, K+ and Cl-).


How can we assist you?

Learn about our ARCHITECT ci8200 services, and how we can assist you in your specific project.


Enzyme-linked immunosorbent assay (ELISA) is a commonly used analytical immunoassay technique involving detection of an analyte normally when there is a demand for measurements on a smaller scale and/or lower sensitivity which can be very cost-effective. Although the radioimmunoassay (RIA) method is similar in principle to the non-radioactive sandwich ELISA method, it uses radiolabeled molecules thereby making it extremely sensitive and extremely specific. This method can be used for any biological molecule in principle and is not restricted to serum or plasma antigens. Because radioactive substances are used it requires special precautions and licensing in combination with specialized equipment. Arcadia is utilizing this technique on a wide variety of projects involving high sensitive screening for hormone levels in blood samples.


How can we assist you?

Learn about our ELISA/RIA services, and how we can assist you in your specific project.


Due to the great flexibility, LC-MS/MS is very suitable for research purposes in patient care. Quantitative analysis in very low detection levels (in pmol/L) is possible for the analysis of endogenous compounds. No matter what type of analysis, endogenous, exogenous compounds or biomarkers it is possible to work with small sample volumes. 

In addition the LC-MS/MS technique is widely applicable in terms of diverse sample material. Serum, plasma, dry blood spot, breast milk, saliva or urine have all been used and well documented. Due to the rapidly evolving field of LC_MS/MS, today Arcadia is able to use this technique to perform targeted proteomics, lipodomics and detection of very small molecules. Because the platform is an open format it enables for custom assay development.


How can we assist you?

Learn about our LCMS services, and how we can assist you in your specific project.

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