Olink

ARCADIA is the official and certified Olink service provider for the BeNeLux countries since 2018. With our Olink proteomics platform we can offer a high capacity analysis service for comprehensive protein biomarker analyses using many different disease-and biological process-focused panels. Using minimal sample volume this provides quantifiable results with highthroughput, exceptional sensitivity and specificity, with coverage across a broad dynamic range.

With our Olink proteomics platform we can offer a high-capacity analysis service for comprehensive protein biomarker analyses using many different disease-and biological process-focused panels. This helps scientists to bring new insights into disease processes, improve disease detection, and contribute to a better understanding of biology. Identifying multiple proteins that form a signature is more powerful and reliable than looking at a single protein.

Our Olink analysis service offers high-capacity analysis of your samples using any of our Olink Target platforms for protein biomarker analysis. With a focus on data quality and customer service we are here to help you accelerate your research.

Let Arcadia run your samples

If you choose to send your sample for our experts to run on the Olink platform, we can assist you with the following:

1. Design of study and assay set up.
2. Sample analysis.
3. Data processing with normalized results and QC report.
4. Delivery of results, usually within 4 weeks.

Analysis Service workflow

Please note that the download below was written principally to describe the Olink Analysis Service workflow for our Target 96, 48 and Flex panels.

Technology

The unique technology behind the Olink® products enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample. PEA technology enables high multiplexing levels and sample throughput without compromising on data or assay-robustness.

A highly specific DNA template is created when matched oligo-linked Ab pairs bind to a protein. The digital signal is read out by qPCR or NGS.

Technology capabilities

Using PEA, Olink® technology can provide accurate quantification below pg/mL levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes our precision proteomics solution especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures. Fully validated in serum & plasma, PEA is also compatible with a wide range of different sample types.

Olink® Explore

Olink® Explore series offers high-throughput proteomics, analysing thousands of proteins simultaneously with exceptional specificity and scalability, enabling advanced disease research and biomarker discovery.

Olink® Explore HT

Next-level proteomics with uncompromised performance. Measure 5,400+ proteins with proven specificity to gain an understanding of disease at the protein level. Realize any project you can imagine. Olink® Explore HT is designed for outstanding performance at any scale of protein biomarker discovery, from pilot investigations with a few hundred samples to the largest population studies with biobank cohorts. Measure ~5,400 protein biomarkers with industry-leading specificity. With the launch of Olink® Explore HT, the assay library was significantly expanded with nearly 2 500 new assays compared to Explore 3072, bringing the total number to more than 5 400 assays. Explore HT has extensive coverage of inflammatory and immune response-related proteins, secreted
proteins, drug targets, disease-associated proteins, and canonical plasma proteins.

Olink Explore 3072

Modular high-multiplex biomarker discovery with uncompromised data quality. Olink Explore 3072 library consists of eight disease- and biological process-focused 384-plex panels. Providing a flexible and efficient solution for larger scale targeted investigations. The assays available in these panels include biomarkers that contribute the most to key research questions and cover many key biological pathways and functions; Cardiometabolic, Inflammation, Neurology and Oncology. Five main categories of proteins were defined during target selection, and each assay can be classified into one or more of these categories:

• Low-abundant inflammation proteins;
• Proteins actively secreted into blood circulation;
• Approved and ongoing drug target proteins;
• Organ-specific proteins that have leaked into blood circulation;
• Proteins representing more exploratory potential biomarkers.

Olink® Reveal

A Powerful, NGS-based proteomics with more than 1000 proteins – uncovering meaningful insights. The protein selection strategy focuses on actionable, druggable, robustly detected circulating proteins with broad proteome coverage, particularly those involved in
inflammation and immune response, drawn from protein annotations, publications, approved therapies, and researcher requests.

Olink® Target

Olink® T96 panels

Each panel is focused on a specific area of disease or key biology process, targeting 92 established and/or exploratory biomarkers. All assays are rigorously quality controlled and our validation data is made freely available. Currently there are six disease area-focus panels available: two for cardiovascular disease (Olink® Target 96 Cardiovascular II and Olink® Target 96 Cardiovascular III), two for  oncology (Olink® Target 96 Oncology II and Olink® Target 96 Oncology III), as well as  Olink® Target 96 Neurology, Olink Target 96 Inflammation and Olink Target 96 Immuno-oncology. These are complemented by seven panels, focused on important biological processes: Olink® Target 96 Cardiometabolic, Olink Target 96 Cell Regulation, Olink® Target 96 Development, Olink® Target 96 Immune Response, Olink® Target 96 Metabolism , Olink Target 96 Neuro Exploratory and Olink® Target 96 Organ Damage. For researchers working with mouse models, Olink® Target 96 Mouse Exploratory is also available.

Olink® T48 panels

Olink® Target 48 Human or Mouse Cytokine panel is the ultimate solution for cytokine studies and inflammation-related diseases. The thorough selection of biomarkers ensures that the most important proteins covering key pathways related to cytokine signalling and inflammatory processes are well covered. 

Target 48 Cytokine enables analysis of 45 carefully selected proteins across 40 samples simultaneously from just 1 µL sample. In comparison to other products for multiple cytokine analysis, this panels offers superior sensitivity, specificity and dynamic range. Absolute quantification in standard concentration units (pg/mL) via provision of calibrators for each assay (first time this is available in a standard Olink panel) Each analyte in the panel has been validated in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference.

Olink® Flex

Olink® Flex is a customizable made-to-order product that enables the selection and combination of up to 21 human proteins into a single protein biomarker panel. Freely mix and match from ~200 pre-validated assays covering major disease areas. Olink® Flex panels offer mid-plex proteomics with unequaled flexibility and absolute quantification to cover a wide range of needs.
Mix and match 15-21 pre-validated assays from an extensive Olink® library across 40 samples simultaneously from just 1 µL sample. Each assay maintains the quality, performance, specificity, and sensitivity that our other products are known for. Our internal controls remove the need to run replicate samples, and the data is reported in absolute (pg/mL) or relative (NPX) quantification.