Olink

ARCADIA is the official and certified Olink service provider for the BeNeLux countries since 2018. With our Olink proteomics platform we can offer a high capacity analysis service for comprehensive protein biomarker analyses using many different disease-and biological process-focused panels. Using minimal sample volume this provides quantifiable results with highthroughput, exceptional sensitivity and specificity, with coverage across a broad dynamic range.

With our Olink proteomics platform we can offer a high capacity analysis service for comprehensive protein biomarker analyses using many different disease-and biological process-focused panels. This helps scientists to bring new insights into disease processes, improve disease detection, and contribute to a better understanding of biology. Identifying multiple proteins that form a signature is more powerful and reliable than looking at a single protein. Each of our well-established 96-plex panels provides assays for 92 different proteins that are focused on a specific disease area or important biological process.

Our Olink analysis service offers high-capacity analysis of your samples using our Olink Target platform for protein biomarker analysis. With a focus on data quality and customer service we are here to help you accelerate your research.

Let Arcadia run your samples

If you choose to send your sample for our experts to run on the Olink platform, we can assist you with the following:

1. Design of study and assay set up.
2. Sample analysis.
3. Data processing with normalized results and QC report.
4. Delivery of results, usually within 4 weeks.

Analysis Service workflow

Please note that the download below was written principally to describe the Olink Analysis Service workflow for our Target 96, 48 and Flex panels.

Technology

The unique technology behind our Olink® Target 96 and Target 48 panels enables high-throughput, multiplex immunoassays of proteins using minimal volumes of serum, plasma, or almost any other type of biological sample. PEA technology enables high multiplexing levels and sample throughput without compromising on data or assay-robustness. 

A highly specific DNA template is created when matched oligo-linked Ab pairs bind to a protein. The digital signal is read out by qPCR

Technology capabilities

Using PEA, our panels can provide accurate quantification below pg/mL levels even in small samples from, for example, needle biopsies or micro dialysis. This minimal sample consumption makes our precision proteomics solution especially suitable for retroactively mining precious biobanked serum samples for new multivariate biomarker signatures. Fully validated in serum & plasma, PEA is also compatible with a wide range of different sample types.

Olink T96 panels

Each panel is focused on a specific area of disease or key biology process, targeting 92 established and/or exploratory biomarkers. All assays are rigorously quality controlled and our validation data is made freely available. Currently there are six disease area-focus panels available: two for cardiovascular disease (Olink Target 96 Cardiovascular II and Olink Target 96 Cardiovascular III), two for  oncology (Olink Target 96 Oncology II and Olink Target 96 Oncology III), as well as  Olink Target 96 Neurology, Olink Target 96 Inflammation and Olink Target 96 Immuno-oncology. These are complemented by seven panels, focused on important biological processes: Olink Target 96 Cardiometabolic, Olink Target 96 Cell Regulation, Olink Target 96 Development, Olink Target 96 Immune Response, Olink Target 96 Metabolism , Olink Target 96 Neuro Exploratory and Olink Target 96 Organ Damage. For researchers working with mouse models, Olink Target 96 Mouse Exploratory is also available.

Olink T48 panels

Olink Target 48 Cytokine is the ultimate solution for cytokine studies and inflammation-related diseases. The thorough selection of biomarkers ensures that the most important proteins covering key pathways related to cytokine signaling and inflammatory processes are well covered. 

Target 48 Cytokine enables analysis of 45 carefully selected proteins across 40 samples simultaneously from just 1 µL sample. In comparison to other products for multiple cytokine analysis, this panels offers superior sensitivity, specificity and dynamic range. Absolute quantification in standard concentration units (pg/mL) via provision of calibrators for each assay (first time this is available in a standard Olink panel) Each analyte in the panel has been validated in terms of specificity, precision, sensitivity, dynamic range, matrix effects and interference.

Olink® Flex

Olink® Flex is a customizable made-to-order product that enables the selection and combination of up to 21 human proteins into a single protein biomarker panel. Freely mix and match from ~200 pre-validated assays covering major disease areas. Olink® Flex panels offer mid-plex proteomics with unequaled flexibility and absolute quantification to cover a wide range of needs.
Mix and match 15-21 pre-validated assays from an extensive Olink library across 40 samples simultaneously from just 1 µL sample. Each assay maintains the quality, performance, specificity, and sensitivity that our other products are known for. Our internal controls remove the need to run replicate samples, and the data is reported in absolute (pg/mL) or relative (NPX) quantification.